{"product_id":"cst-12816t","title":"CST,  12816T, SSU72 (D3I2D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SSU72. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, SSU72 (D3I2D) Rabbit Monoclonal Antibody (CST #12816) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor more information about the RBP-eCLIP service please visit Eclipsebio .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\neCLIP: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSSU72 (D3I2D) Rabbit Monoclonal Antibody recognizes endogenous levels of total SSU72 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro93 of human SSU72 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSSU72 is a protein phosphatase originally identified through its interaction with TFIIB as a factor required for transcription and RNA processing (1,2). While SSU72 has been known as a phosphatase of the RNA polymerase II carboxy-terminal domain at Ser5 of the heptapeptide repeat, additional studies suggest a role for SSU72 as a phosphatase for Ser7 of the heptapeptide repeat as well (3-9). The activities of SSU72 are thought to play a critical role in coupling transcription with pre-mRNA 3' end processing (10-12). SSU72 is required for gene looping that connects the gene promoter with the terminator region, leading to proper initiation of transcription and enforcement of transcriptional directionality on promoters that may other wise act as bidirectional promoters (13-14). In addition to a role in transcription, SSU72 acts as a cohesin binding protein that regulates cohesion between sister chromatid arms during mitosis or meiosis (15).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCTD phosphatase SSU72; FLJ13048; HSPC182; PNAS-120; RNA polymerase II subunit A C-terminal domain phosphatase SSU72; SSU72; SSU72 homolog, RNA polymerase II CTD phosphatase; Ssu72 RNA polymerase II CTD phosphatase homolog; SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae)\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 27\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797581222057,"sku":"12816T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12816t","provider":"Iright","version":"1.0","type":"link"}