CST, 12818S, Phospho-CaMKK2 (Ser511) Antibody

Polyclonal Antibody for studying CaMKK2 (Ser511) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-CaMKK2 (Ser511) Antibody (CST #12818) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-CaMKK2 (Ser511) Antibody recognizes endogenous levels of CaMKK2 protein only when phosphorylated at Ser511. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser511 of human CaMKK2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Calcium/Calmodulin-dependent Protein Kinase Kinase 2 (CaMKK2) is a member of the CaMK family that contains a central Ser/Thr kinase domain followed by a regulatory domain consisting of overlapping autoinhibitory and CaM-binding regions (1). CaMKK2 can be distinguished from other CaMK family members by the presence of a unique Pro/Arg/Gly-rich insert following the ATP-binding domain (2). CaMKK2 phosphorylates CaMKI at Thr177 and CaMKIV at Thr200 (3). CaMKK2 also phosphorylates AMPKα in response to calcium (4). CaMKK2 has been implicated in long-term memory formation (5) and adipocyte development (6). CaMKK2 is phosphorylated at Ser511 by death-associated protein kinase (DAPK) in a signaling cascade thought to be involved in neuronal death (7). Alternate Names calcium/calmodulin dependent protein kinase kinase 2; calcium/calmodulin-dependent protein kinase beta; Calcium/calmodulin-dependent protein kinase kinase 2; calcium/calmodulin-dependent protein kinase kinase 2, beta; Calcium/calmodulin-dependent protein kinase kinase beta; CaM-kinase kinase 2; CaM-kinase kinase beta; CaM-KK 2; CaM-KK beta; CAMKK; CaMKK 2; CaMKK beta; CAMKK beta protein; CAMKK2; CAMKKB; KIAA0787; KKCC2; MGC15254 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 68 SOURCE: Rabbit