CST, 12831S, TAL1 Antibody

Polyclonal Antibody for studying TAL1. Validated for Western Blotting. Highly specific and rigorously validated in-house, TAL1 Antibody (CST #12831) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TAL1 Antibody recognizes transfected levels of total TAL1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human TAL1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The basic helix-loop-helix (bHLH) family of transcription factors play roles in a variety of developmental and adult biological settings (1). The basic (b) domain of bHLH transcription factors allows association with DNA, while the HLH domain facilitates the mono- and heterodimeric interactions required for efficient DNA binding (1). TAL1, also known as SCL, is a bHLH transcription factor that plays an essential role in the generation of embryonic hematopoietic stem cells (2), and is required for maturation of erythroid and megakaryocytic lineages in the adult (3). TAL1 interacts with Lim-domain containing proteins LMO2 and Ldb1, and has been found in complexes with these proteins as well as the hematopoietic transcription factor GATA-1 (4). Both TAL1 and LMO2 have been associated with T-cell acute lymphoblastic leukemia (T-ALL) (5,6). TAL1 activity is essential for both hematopoiesis and neurogenesis (7). Alternate Names BHLHA17; Class A basic helix-loop-helix protein 17; SCL; Stem cell protein; T-cell acute lymphocytic leukemia 1; T-cell acute lymphocytic leukemia protein 1; T-cell leukemia/lymphoma protein 5; TAL bHLH transcription factor 1, erythroid differentiation factor; TAL-1; tal-1 product; TAL1; TCL5 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 50 SOURCE: Rabbit