CST, 12844S, DNAJC2/MPP11 (D6B1E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DNAJC2. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, DNAJC2/MPP11 (D6B1E) Rabbit Monoclonal Antibody (CST #12844) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity DNAJC2/MPP11 (D6B1E) Rabbit Monoclonal Antibody recognizes endogenous levels of total DNAJC2/MPP11 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro540 of human DNAJC2/MPP11 protein. Background DnaJ/Hsp40 proteins are a conserved family of J-domain-containing chaperone proteins that assist in protein folding and stability through their interactions with Hsp70 chaperone proteins (reviewed in 1). DNAJC2, also known as MPP11 (M-phase phosphoprotein 11 protein) or ZRF1, is a component of the ribosome-associated complex (RAC). The RAC is localized to the cytoplasm, where it assists in maintaining appropriate folding of nascent polypeptides by stimulating the ATPase activity of Hsp70 chaperone proteins (2,3). In the nucleus, MPP11 is involved in the activation of transcription through mediation of the switch from polycomb-repressed to active chromatin (4). Previous studies have shown MPP11 is overexpressed in leukemia and head and neck cancer, leading researchers to suggest MPP11 may be a potential therapeutic target (5-7). MPP11 is phosphorylated at serine 47 by S6 kinase, which regulates senescence in fibroblast cells (8). Alternate Names DnaJ (Hsp40) homolog, subfamily C, member 2; DnaJ heat shock protein family (Hsp40) member C2; DnaJ homolog subfamily C member 2; DnaJ homolog subfamily C member 2, N-terminally processed; DNAJC2; DNJC2; M-phase phosphoprotein 11; MPHOSPH11; MPP11; ZRF1; ZUO1; zuotin related factor 1; Zuotin-related factor 1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG