CST, 12889S, hERG1a (D1Y2J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying hERG. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, hERG1a (D1Y2J) Rabbit Monoclonal Antibody (CST #12889) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity hERG1a (D1Y2J) Rabbit Monoclonal Antibody recognizes endogenous levels of both mature and immature hERG1a protein. This antibody cross-reacts with proteins of unknown origin at 65 and 42 kDa in some cell lines. This antibody does not recognize hERG1b protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala223 of human hERG1a protein. Background hERG1(human ether-a-go-go-related gene potassium channel 1) is a voltage gated potassium channel alpha-subunit which mediates the rapidly activating component of the delayed rectifying potassium current in heart (IKr) (1,2). The hERG channel is composed of two subunits, 1a and 1b, which differ at amino terminus due to alternative splicing. Native hERG channels are heteromers of hERG1a with hERG1b. Both subunits contribute to IKr current (3-6). Blockade of hERG currents induced by compounds or mutation of hERG encoding gene-KCNH2 causes ventricular arrhythmias associated with inherited and acquired long QT syndrome and cardiomyocyte apoptosis (7-10). Therefore, hERG channel is a primary target for the development of class III antiarrhythmic agents (11,12). The hERG channel is also inhibited by a variety of non-antiarrhythmic compounds, which result in side effects. Consequently, hERG channel blockage is a common counter screen when selecting therapeutic agents for various diseases (11,13,14). Research studies have implicated hERG in cancer cell survival (15). In normal human adult tissue, hERG is expressed in heart, brain, myometrium, pancreas, and hematopoietic progenitors (16,17). hERG is expressed in various cancer cell lines of epithelial, neuronal, leukemic, and connective tissue origin but not in corresponding normal cells (18-22). Furthermore, hERG expression is associated with enhanced cancer cell proliferation, invasiveness, and poor prognosis (23,24). Alternate Names Eag homolog; Eag-related protein 1; ERG; ERG-1; ERG1; Ether-a-go-go-related gene potassium channel 1; ether-a-go-go-related potassium channel protein; Ether-a-go-go-related protein 1; H-ERG; HERG; hERG-1; hERG1; human ether-a-go-go-related; KCNH2; Kv11.1; long QT syndrome type 2; LQT2; potassium channel, voltage gated eag related subfamily H, member 2; Potassium voltage-gated channel subfamily H member 2; potassium voltage-gated channel, subfamily H (eag-related), member 2; SQT1; Voltage-gated potassium channel subunit Kv11.1; voltage-gated potassium channel, subfamily H, member 2 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 135, 155 Source/Isotype: Rabbit IgG