{"product_id":"cst-12891s","title":"CST,  12891S, SIX1 (D4A8K) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SIX1. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, SIX1 (D4A8K) Rabbit Monoclonal Antibody (CST #12891) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:100 - 1:400\nFlow Cytometry (Fixed\/Permeabilized): 1:400 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 81173 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSIX1 (D4A8K) Rabbit Monoclonal Antibody recognizes endogenous levels of total SIX1 protein. This antibody does not cross-react with other SIX family proteins.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro249 of human SIX1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSine oculis homeobox (SIX) proteins belong to a family of evolutionarily conserved transcription factors discovered in mutant screens for embryonic eye development genes (1-3). The prototypical family member ( , ) was named for eyeless embryos carrying mutations in a gene highly conserved among vertebrates, including humans ( ) (4). A total of six family members (SIX1-6) have been identified in vertebrates. Each SIX protein contains a homeobox nucleic acid recognition domain (HD) with a DNA-binding helix-turn-helix motif and an adjacent SIX domain, which may be involved in regulating protein-protein interactions (5). In addition to their critical functions during embryonic organogenesis, research studies suggest that SIX proteins play additional roles in postnatal cell cycle regulation, with potentially important implications in tumorigenesis (6,7). In contrast to the ortholog, the vertebrate gene product does not play a critical role in embryonic eye development. Vertebrate SIX1 is required for development of mesoderm- and neural crest-derived lineages, and male reproductive tissues (8-10). SIX1 has also been shown to regulate transcription of MyoD in adult muscle progenitor cells during postnatal muscle development (11). A mechanistic role for SIX1 in cell cycle regulation is supported by research studies showing increased SIX1 expression in various cancer subtypes, including breast, ovarian, and hepatocellular carcinoma (6,12,13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBOS3; DFNA23; Homeobox protein SIX1; Sine oculis homeobox homolog 1; SIX homeobox 1; SIX1; TIP39\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 36\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797582860457,"sku":"12891S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12891s","provider":"Iright","version":"1.0","type":"link"}