CST, 12892S, SCAI (D9A7Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SCAI. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SCAI (D9A7Y) Rabbit Monoclonal Antibody (CST #12892) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SCAI (D9A7Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total SCAI protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SCAI protein. Background The serum response factor (SRF) is a ubiquitous protein that modulates transcription of genes containing serum response elements (SRE) at their promoters. SRF regulates cellular processes such as cell proliferation and cytoskeletal signaling in conjunction with a variety of cofactors (1-3). Suppressor of cancer cell invasion (SCAI) is a highly conserved transcriptional cofactor that inhibits the activity of myocardin-related transcription factor (MRTF) family members MAL (MRTF-A), myocardin and OTT-MAL (4,5). SCAI controls the expression of integrin β1 and regulates cell migration and invasion (4). SCAI has also been shown to play a role in transcriptional regulation in neurons by regulating dendritic morphology through inhibition of the megakaryoblastic leukemia (MKL) family of transcription cofactors (6). Research studies have implicated SCAI in the regulation of the epithelial-to-mesenchymal transition (EMT) as well as in renal fibrosis (7). Alternate Names C9orf126; FLJ11758; FLJ36664; MGC120525; MGC120526; MGC120528; NET40; Protein SCAI; SCAI; suppressor of cancer cell invasion; Suppressor of cancer cell invasion protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG