CST, 12906S, Dab2 (D7O9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DAB2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Dab2 (D7O9T) Rabbit Monoclonal Antibody (CST #12906) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 98694 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Dab2 (D7O9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total Dab2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly388 of human Dab2 protein. Background Disabled homolog 2 (Dab2) is a mitogen responsive phosphoprotein that exerts multiple functions through association with numerous proteins. Dab2 modulates signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4), and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Research studies have shown Dab2 is downregulated in a number of cancers, thereby suggesting a role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18). Alternate Names Adaptor molecule disabled-2; DAB adaptor protein 2; Dab, mitogen-responsive phosphoprotein, homolog 2; DAB2; DAB2, clathrin adaptor protein; Differentially expressed in ovarian carcinoma 2; Differentially-expressed protein 2; Disabled homolog 2; disabled homolog 2, mitogen-responsive phosphoprotein; disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila); DOC-2; DOC2; FLJ26626; mitogen-responsive phosphoprotein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 96 Source/Isotype: Rabbit IgG