{"product_id":"cst-12930s","title":"CST,  12930S, K63-linkage Specific Polyubiquitin (D7A11) Rabbit Monoclonal Antibody (HRP Conjugate)","description":"Monoclonal Antibody for studying . Validated for Western Blotting. Highly specific and rigorously validated in-house, K63-linkage Specific Polyubiquitin (D7A11) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #12930) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nK63-linkage Specific Polyubiquitin (D7A11) Rabbit Monoclonal Antibody (HRP Conjugate) detects polyubiquitin chains formed by Lys63 residue linkage. This antibody conjugate does not react with monoubiquitin or polyubiquitin chains formed by linkage to a different lysine residue.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the Lys63 branch of the human diubiquitin chain.\n\u003cb\u003eBackground\u003c\/b\u003e\nUbiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800338649257,"sku":"12930S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12930s","provider":"Iright","version":"1.0","type":"link"}