CST, 12953T, Gelsolin (D9W8Y) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying gelsolin. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Gelsolin (D9W8Y) Rabbit Monoclonal Antibody (CST #12953) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 57376 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Gelsolin (D9W8Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total gelsolin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val377 of human gelsolin protein. Background Gelsolin (actin-depolymerizing factor, ADF, AGEL, Brevin) is an 83 kDa protein that shares structural and functional homology to villin and adseverin/scinderin (1,2). Gelsolin plays an important role in actin filament assembly by capping and severing actin proteins in a Ca -dependent manner (3,4). Gelsolin is important for cellular events (e.g., membrane ruffling, chemotaxis, ciliogenesis) that require cytoskeletal remodeling (3). Accordingly, cells from gelsolin knockout mice exhibit motility defects, including a failure to ruffle in response to growth factor stimulation (5,6). In humans, defects in gelsolin have been linked to amyloidosis type 5 (AMYL5), a hereditary disease characterized by cranial neuropathy, which appears to result from gelsolin amyloid deposition (7). Alternate Names Actin-depolymerizing factor; ADF; AGEL; Brevin; DKFZp313L0718; epididymis secretory sperm binding protein; GELS; Gelsolin; gelsolin (amyloidosis, Finnish type); GSN Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 83 Source/Isotype: Rabbit IgG