{"product_id":"cst-12963s","title":"CST,  12963S, Cytochrome c (6H2.B4) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying cytochrome C. Validated for Immunoprecipitation,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, Cytochrome c (6H2.B4) Mouse Monoclonal Antibody (CST #12963) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:300\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCytochrome c (6H2.B4) Mouse Monoclonal Antibody recognizes endogenous levels of total cytochrome c protein by immunofluorescence. This antibody is not recommended for western blot.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant rat cytochrome c protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)\/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCYC; CYCS; Cytochrome c; cytochrome c, somatic; HCS; THC4\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 14\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800247193769,"sku":"12963S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12963s","provider":"Iright","version":"1.0","type":"link"}