CST, 12974S, FoxC2 (D4D4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FoxC2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, FoxC2 (D4D4) Rabbit Monoclonal Antibody (CST #12974) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FoxC2 (D4D4) Rabbit Monoclonal Antibody recognizes endogenous levels of total FoxC2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu278 of human FoxC2 protein. Background Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA-binding domain called a Forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech/language disorders, and cancer (3,4). The expression of FoxC2 and several other Fox transcription factors are regulated during adipogenesis, suggesting their involvement in adipocyte differentiation (5). A transgenic mouse model exhibiting over expression of FoxC2 in white adipose tissue (WAT) and brown adipose tissue (BAT) showed a reduction of the intra-abdominal WAT depot (6). The BAT-specific gene Ucp1 is upregulated in the intra-abdominal WAT depot of these transgenic mice, resulting in histological features similar to those seen in BAT. This evidence suggests that FoxC2 can play a protective role against obesity and diet-induced insulin resistance (6). Alternate Names FKHL14; forkhead box C2; forkhead box C2 (MFH-1, mesenchyme forkhead 1); Forkhead box protein C2; Forkhead-related protein FKHL14; forkhead, Drosophila, homolog-like 14; FOXC2; LD; Mesenchyme fork head protein 1; mesenchyme forkhead 1; MFH-1; MFH-1 protein; MFH-1,mesenchyme forkhead 1; MFH1; Transcription factor FKH-14 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG