CST, 12985S, PSMD10/Gankyrin Antibody

Polyclonal Antibody for studying PSMD10. Validated for Western Blotting. Highly specific and rigorously validated in-house, PSMD10/Gankyrin Antibody (CST #12985) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PSMD10/Gankyrin Antibody recognizes endogenous levels of total PSMD10/Gankyrin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PSMD10/Gankyrin protein. Antibodies are purified by protein A and peptide affinity chromatography. Background PSMD10/Gankyrin is an ankyrin-repeat chaperone protein that is involved in the assembly of the 19S regulatory particle of the proteasome (1). Reasearch studies have demonstrated that PSMD10 is oncogenic and is overexpressed in hepatocullar carcinomas (2). Investigators believe the oncogenicity of PSMD10 may be linked to its ability to bind and regulate the stability and activity of pRB, CDK4, MDM2, and RelA (2-5). Alternate Names 26S proteasome non-ATPase regulatory subunit 10; 26S proteasome regulatory subunit p28; ankyrin repeat protein; dJ889N15.2; Gankyrin; hepatocellular carcinoma-associated protein p28-II; p28; p28(GANK); proteasome (prosome, macropain) 26S subunit, non-ATPase, 10; proteasome 26S non-ATPase subunit 10; proteasome 26S subunit, non-ATPase 10; PSD10; PSMD10 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 SOURCE: Rabbit