{"product_id":"cst-12993s","title":"CST,  12993S, Spry1 (D9V6I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Spry1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Spry1 (D9V6I) Rabbit Monoclonal Antibody (CST #12993) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSpry1 (D9V6I) Rabbit Monoclonal Antibody recognizes endogenous levels of total Spry1 protein. This antibody may also cross-react with an unidentified protein at 65 kDa.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val145 of human Spry1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSpry1 is a member of the Sprouty (Spry) family proteins that was initially identified in as an inhibitor of the FGF signaling pathway (1). There are four human Spry proteins (Spry1-4), encoded by different genes, and they all share a highly conserved carboxy-terminal cystine-rich Spry domain that is known to be essential for their receptor tyrosine kinase inhibitory function stimulated by various growth factors (1-3). Spry1 and other Spry proteins play a key role in embryonic development, tissue and organ formation, as well as growth in almost all living organisms (1-4). Spry proteins are considered tumor suppressors due to their inhibitory function in a variety of growth factor signaling pathways (2,3). Spry1 anchors itself to the membrane by palmitoylation and can translocate from the cytosol to the membrane by binding to caveolin-1 (5,6). Regulation of Spry1 protein function is thought to occur at various levels. Spry1 regulation includes transcriptional regulation by growth factors and kinases (1,4,7), post-transcriptional regulation by microRNA-21 (8), post-translational modifications including phosphorylation, dephosphorylation, ubiquitination and proteasomal degradation, and regulation by its interacting protein partners (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nhSPRY1; Protein sprouty homolog 1; sprouty homolog 1, antagonist of FGF signaling; sprouty homolog 1, antagonist of FGF signaling (Drosophila); sprouty RTK signaling antagonist 1; sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling); Spry-1; SPRY1; SPY1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 35\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797584662697,"sku":"12993S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-12993s","provider":"Iright","version":"1.0","type":"link"}