CST, 13029S, PFKFB2 (D5I5F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PFK-2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, PFKFB2 (D5I5F) Rabbit Monoclonal Antibody (CST #13029) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity PFKFB2 (D5I5F) Rabbit Monoclonal Antibody recognizes endogenous levels of total PFKFB2 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val82 within the 6-phosphofructo-2-kinase domain of human PFKFB2 protein. Background The bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). Research studies indicate that amino acids activate PFKFB2 through Akt-dependent phosphorylation at Ser483 on PFKFB2 (3). In addition, androgen increases the expression of PFKFB2 in prostate cancer cells (4). Alternate Names 6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; 6PF-2-K/Fru-2,6-P2ase heart-type isozyme; DKFZp781D2217; F262; Fructose-2,6-bisphosphatase; fructose-2,6-bisphosphatase, cardiac isozyme; MGC138308; MGC138310; PFK-2/FBPase-2; PFK/FBPase 2; PFKFB, cardiac; PFKFB2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG