{"product_id":"cst-13045t","title":"CST,  13045T, PFKFB2 (D7G5R) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PFK-2. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, PFKFB2 (D7G5R) Rabbit Monoclonal Antibody (CST #13045) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPFKFB2 (D7G5R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PFKFB2 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro454 within the fructose-2,6-biphosphatase region of human PFKFB2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe bifunctional 6-phosphofructo-2-kinase\/fructose-2,6-bisphosphatase (PFK\/FBPase or PFKFB) catalyzes the synthesis and degradation of fructose 2,6-bisphosphate and regulates its steady-state level (1,2). Fructose 2,6-bisphosphate activates phosphofructokinase, a rate-limiting enzyme in glycolysis, by allosteric regulation (1,2). Four different PFKFB isoforms (PFKFB1, PFKFB2, PFKFB3, and PFKFB4) have been identified (1,2). Research studies indicate that amino acids activate PFKFB2 through Akt-dependent phosphorylation at Ser483 on PFKFB2 (3). In addition, androgen increases the expression of PFKFB2 in prostate cancer cells (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase\/fructose-2,6-biphosphatase 2; 6-phosphofructo-2-kinase\/fructose-2,6-bisphosphatase 2; 6PF-2-K\/Fru-2,6-P2ase 2; 6PF-2-K\/Fru-2,6-P2ase heart-type isozyme; DKFZp781D2217; F262; Fructose-2,6-bisphosphatase; fructose-2,6-bisphosphatase, cardiac isozyme; MGC138308; MGC138310; PFK-2\/FBPase-2; PFK\/FBPase 2; PFKFB, cardiac; PFKFB2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797585776809,"sku":"13045T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13045t","provider":"Iright","version":"1.0","type":"link"}