CST, 13053S, STAM1 Antibody

Polyclonal Antibody for studying STAM1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, STAM1 Antibody (CST #13053) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity STAM1 Antibody recognizes endogenous levels of total STAM1 protein. This antibody does not cross-react with STAM2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp286 of human STAM1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Signal Transducing Adaptor Molecule 1 (STAM1) is a ubiquitously expressed adaptor protein containing an SH3 domain and an ITAM motif. Initial research studies demonstrated that STAM1 undergoes tyrosine phosphorylation following treatment with numerous cytokines and growth factors (1). Subsequent research studies identified STAM1 as a component of the ESCRT-0 complex, which mediates the endocytic sorting of ubiquitinated membrane proteins to the lysosomal compartment for degradation (2). STAM1 harbors a tandemly-oriented VHS (Vps27/Hrs/STAM) domain and UIM (ubiquitin-interacting motif) that facilitates STAM1 binding to ubiquitinated cargo proteins within the endosomal compartment (3,4). Gene targeting studies have revealed that STAM1 and STAM2 cooperate to promote thymic T-cell development and survival (5). Alternate Names DKFZp686J2352; HSE1 homolog; Signal transducing adapter molecule 1; signal transducing adaptor molecule; signal transducing adaptor molecule (SH3 domain and ITAM motif) 1; STAM; STAM-1; STAM1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 SOURCE: Rabbit