CST, 13062S, AGR2 (D9V2F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying AGR2. Validated for WB,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, AGR2 (D9V2F) Rabbit Monoclonal Antibody (CST #13062) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:800 Immunofluorescence (Immunocytochemistry): 1:100 Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 86533 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity AGR2 (D9V2F) Rabbit Monoclonal Antibody recognizes endogenous levels of total AGR2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala29 of human AGR2 protein. Background Anterior gradient homolog 2 (AGR2) is a member of the protein disulfide isomerase (PDI) family of proteins and a homolog of the cement gland protein (1). In normal human tissues, AGR2 is expressed most abundantly in intestinal cells. Research studies have found AGR2 is overexpressed in a number of adenocarcinomas, including those derived from breast, pancreas, ovary, prostate and esophagus (2-4). and studies have shown that AGR2 positively regulates cell growth and division, while its overexpression can promote cell transformation (5,6). The latter functions of AGR2 were shown to involve YAP1-mediated up-regulation of amphiregulin expression, implicating AGR2 in both the EGF and Hippo kinase signaling pathways (6). Alternate Names AG-2; AG2; AGR2; anterior gradient 2 homolog; anterior gradient 2, protein disulphide isomerase family member; anterior gradient homolog 2; anterior gradient homolog 2 (Xenopus laevis); Anterior gradient protein 2 homolog; epididymis secretory protein Li 116; GOB-4; hAG-2; HEL-S-116; HPC8; PDIA17; protein disulfide isomerase family A, member 17; secreted cement gland homolog; Secreted cement gland protein XAG-2 homolog; XAG-2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG