CST, 13071S, Symplekin (D5Y3T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying symplekin. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Symplekin (D5Y3T) Rabbit Monoclonal Antibody (CST #13071) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Symplekin (D5Y3T) Rabbit Monoclonal Antibody recognizes endogenous levels of total symplekin protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human symplekin protein. Background Symplekin is necessary for 3'-end cleavage and polyadenylation of mRNAs, histone 3'-end processing and polyadenylation in the cytoplasm (1-5). It is thought to act as a scaffolding protein that brings together factors involved in mRNA 3'-end processing (1,2). Symplekin also plays a role in transcription initiation and termination by RNA polymerase II (RNAPII) through bridging the interaction between the polyadenylation machinery and RNAPII (3,6,7). In addition to its role in mRNA 3'-end processing, research studies have shown that symplekin localizes at epithelial cell tight junctions where it may help to regulate tight junction assembly, thereby maintaining the integrity of the epithelial monolayer and cell polarity (8,9). Alternate Names FLJ27092; SPK; SYM; SYMPK; Symplekin Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 141 Source/Isotype: Rabbit IgG