{"product_id":"cst-13083t","title":"CST,  13083T, Troponin I (D6F8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Troponin-1\/TNNI3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Troponin I (D6F8) Rabbit Monoclonal Antibody (CST #13083) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTroponin I (D6F8) Rabbit Monoclonal Antibody recognizes endogenous levels of total troponin I protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human troponin I protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nTroponin, working in conjunction with tropomyosin, functions as a molecular switch that regulates muscle contraction in response to changes in the intracellular Ca concentration. Troponin consists of three subunits: the Ca -binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca sensitivity by reducing the Ca binding affinity of TnC (1-3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCardiac troponin I; cardiomyopathy, dilated 2A (autosomal recessive); CMD1FF; CMD2A; CMH7; cTnI; familial hypertrophic cardiomyopathy 7; MGC116817; RCM1; TNNC1; TNNI3; troponin I type 3 (cardiac); Troponin I, cardiac muscle; troponin I3, cardiac type\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797586759849,"sku":"13083T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13083t","provider":"Iright","version":"1.0","type":"link"}