CST, 13115S, UVRAG (D2Q1Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UVRAG. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, UVRAG (D2Q1Z) Rabbit Monoclonal Antibody (CST #13115) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UVRAG (D2Q1Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total UVRAG protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly502 of human UVRAG protein. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). It is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. These proteins are involved in the formation of cytoplasmic vacuoles called autophagosomes that are delivered to lysosomes for degradation.The class III type phosphoinositide 3-kinase (PI3KC3)/Vps34 regulates vacuolar trafficking as well as autophagy (4,5). Multiple proteins have been shown to be associated with Vsp34, including: p105/Vsp15, Beclin-1, UVRAG, Atg14, and Rubicon, which can determine Vsp34 function (6-11). UVRAG (UV radiation resistance-associated gene) is associated with the Beclin-1/PI3KC3 complex and promotes PI3KC3 enzymatic activity and autophagy, while suppressing proliferation (11). Beclin-1 binding to UVRAG promotes both autophagosome maturation and endocytic trafficking (6). UVRAG is also a potential tumor suppressor protein with frameshift mutations observed in colon and gastric carcinomas (12,13). Alternate Names beclin 1 binding protein; DHTX; disrupted in heterotaxy; p63; UV radiation resistance associated; UV radiation resistance associated gene; UV radiation resistance-associated gene protein; UVRAG; VPS38 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG