CST, 13141T, SOD2 (D3X8F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SOD2. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, SOD2 (D3X8F) Rabbit Monoclonal Antibody (CST #13141) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 30204 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity SOD2 (D3X8F) Rabbit Monoclonal Antibody recognizes endogenous levels of total SOD2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SOD2 protein. Background Manganese superoxide dismutase (MnSOD or SOD2) is a mitochondrial detoxification enzyme that catalyzes the conversion of superoxide to hydrogen peroxide (1,2). Hydrogen peroxide is then decomposed to water by catalase, glutathione peroxidase, or peroxiredoxins (2). MnSOD/SOD2 and other enzymes involved in antioxidant defense protect cells from reactive oxygen species (ROS) (2). Calorie restriction leads to SIRT3-mediated deacetylation of MnSOD/SOD2 and the subsequent increase of its antioxidant activity (3). MnSOD/SOD2 also plays an essential role in mediating the protective effect of mTOR inhibition to reduce epithelial stem cell senescence (4). Alternate Names epididymis secretory sperm binding protein; gastric cancer-associated lncRNA 1; GClnc1; indophenoloxidase B; IPO-B; IPOB; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn superoxide dismutase; Mn-SOD; MNSOD; MVCD6; SOD2; SODM; Superoxide dismutase [Mn], mitochondrial; superoxide dismutase 2; superoxide dismutase 2, mitochondrial Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG