{"product_id":"cst-13176s","title":"CST,  13176S, Calbindin (D1I4Q) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Calbindin. Validated for WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Calbindin (D1I4Q) Rabbit Monoclonal Antibody (CST #13176) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:800\nImmunofluorescence (Frozen): 1:200\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 46343\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCalbindin (D1I4Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total calbindin protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse calbindin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCalcium-binding proteins of different subfamilies regulate the second messenger calcium. Calbindin, calmodulin, S-100, parvalbumin, and troponin C are members of the low molecular weight calcium-binding protein family (1). Calbindin is expressed in discrete neuronal populations within the CNS and is thought to act as an intracellular calcium buffering protein. Most Purkinje cells express calbindin, which is expressed when neurons start to migrate and differentiate. In contrast, other calcium buffering proteins, such as parvalbumin, are expressed later during development and in parallel with increasing neuronal activity (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCAB27; CALB; CALB1; Calbindin; calbindin 1; calbindin 1, (28kD); calbindin 1, 28kDa; calbindin 27; Calbindin D28; D-28K; RTVL-H protein; Vitamin D-dependent calcium-binding protein, avian-type\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 28\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797589020841,"sku":"13176S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13176s","provider":"Iright","version":"1.0","type":"link"}