{"product_id":"cst-13186t","title":"CST,  13186T, IL-17F (D3M4D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying IL17F mouse. Validated for Western Blotting,Flow Cytometry (Fixed\/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, IL-17F (D3M4D) Rabbit Monoclonal Antibody (CST #13186) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nFlow Cytometry (Fixed\/Permeabilized): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIL-17F (D3M4D) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse IL-17F protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser93 of mouse IL-17F protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe IL-17 family of cytokines consists of IL-17A-F, and their receptors include IL-17RA-RE (1). IL-17 cytokines are produced by a variety of cell types including the Th17 subset of CD4+ T cells, as well as subsets of γδ T cells, NK cells, and NKT cells (2). IL-17A and IL-17F, the most well-studied of the IL-17 cytokines, contribute to fungal and bacterial immunity by inducing expression of proinflammatory cytokines, chemokines, and antimicrobial peptides (2). In addition, IL-17A contributes to the pathogenesis of several autoimmune diseases (3). IL-17E promotes Th2 cell responses (4). The roles of IL-17B, IL-17C, and IL-17D are less clear, however these family members also appear to have the capacity to induce proinflammatory cytokines (1,5,6). IL-17 receptors have an extracellular domain, a transmembrane domain, and a SEFIR domain. They are believed to signal as homodimers, heterodimers, or multimers through their SEFIR domain by recruiting the SEFIR domain-containing adaptor Act1 (7). Unlike most cytokines that signal through Jak\/STAT pathways, IL-17 signaling results in NF-κB activation (8). IL-17F is a cysteine-linked proinflammatory cytokine that can dimerize with itself or form heterodimers with the IL-17 family member that it shares 50% homology with, IL-17A (9). Although mainly produced by Th17 cells, IL-17F expression has been observed in several cell types including activated CD8+ T cells, γδ T cells, NKT cells, B cells and LTi cells. IL-17F binds to a heterodimeric receptor consisting of IL-17RA and IL-17RC, which upon binding induces the TRAF6-mediated activation of TAK and the Erk1\/2 MAP kinase pathway (10). This induces the expression of numerous inflammatory chemokines and cytokines including IL-1β, IL-6, IL-8, and MIP-1β along with increased adhesion molecule expression in human airway epithelial cells, vein endothelial cells, and fibroblasts (11). IL-17F has been linked with asthma and other autoimmune diseases including rheumatoid arthritis, multiple sclerosis, psoriasis, and inflammatory bowel disease (12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC87042; IL-17F; Il17f; interleukin 17F; Interleukin-17F\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 18\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801046667433,"sku":"13186T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13186t","provider":"Iright","version":"1.0","type":"link"}