CST, 13187S, BAP1 (D1W9B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BAP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BAP1 (D1W9B) Rabbit Monoclonal Antibody (CST #13187) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BAP1 (D1W9B) Rabbit Monoclonal Antibody recognizes endogenous levels of total BAP1 protein. This antibody also cross-reacts with an unidentified protein of 42 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys275 within the linker region of human BAP1 protein. Background BRCA1-Associated Protein 1 (BAP1) was originally identified as a BRCA1 associated, nuclear localized ubiquitin hydrolase that suppresses cell growth (1). The protein belongs to the UCH family of deubiquitinases, with a UCH domain in its amino-terminal segment and a BRCA1 interaction domain as well as a nuclear localization signal in its carboxy-terminal segment (1). Frequent gene locus rearrangement, deletion, and null mutation of BAP1 have been found in lung and breast cancers (1,2). mutation analysis of cancer cell line survival and animal tumorigenesis indicates that both the deubiquitinase activity and the nuclear localization signal are required for BAP1 function as a tumor suppressor (3). BAP1 does not have direct deubiquitination activity towards the autoubiquitinated BRCA1/BARD1 E3 complex (4), but its interaction with BARD1 inhibits BRCA1/BARD1 E3 activity by interfering with the complex dimerization process (5). In addition to its interaction with BRCA1/BARD1, BAP1 has also been shown to interact with and deubiquitinate HCF-1, thereby controlling its stability (6). Alternate Names BAP1; BRCA1 associated protein 1; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); BRCA1-associated protein 1; Cerebral protein 6; cerebral protein-13; cerebral protein-6; DKFZp686N04275; FLJ35406; FLJ37180; HUCEP-13; hucep-6; KIAA0272; ubiquitin carboxy-terminal hydrolase; Ubiquitin carboxyl-terminal hydrolase BAP1; UCHL2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG