CST, 13241T, CD46 (D6N7H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD46. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD46 (D6N7H) Rabbit Monoclonal Antibody (CST #13241) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 28704 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD46 (D6N7H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD46 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro199 of human CD46 protein. Background Complement Regulatory Protein; Membrane Cofactor Protein (CD46) is a type 1 membrane protein that plays an important inhibitory role in the complement system (1). CD46 exhibits a cofactor activity that promotes inactivation of C3b and C4b by serum factor 1, thereby protecting host (self) cells from complement-dependent cytotoxicity (1,2). The importance of CD46 to complement regulation is underscored by the observation that genetic loss of CD46 leads to development of atypical hemolytic-uremic syndrome (aHUS), a disease characterized by uncontrolled complement activation (2,3). In addition to its role in complement inactivation, CD46 can function as a receptor for selected bacteria and viruses (4), and is reportedly required for proper fusion of spermatozoa to the oocyte membrane during fertilization (5). CD46 is implicated in the development and/or progression of selected cancer types. For example, research studies show elevated CD46 expression in medulloblastoma tumor samples (6), while CD46 expression has been linked with poor prognosis in breast cancer (7). It has been suggested that upregulation of CD46 may serve to protect cancer cells from complement-dependent cytotoxicity, thereby evading destruction by the immune system (8,9). Alternate Names AHUS2; antigen identified by monoclonal antibody TRA-2-10; CD46; CD46 antigen, complement regulatory protein; CD46 molecule; CD46 molecule, complement regulatory protein; complement membrane cofactor protein; MCP; measles virus receptor; Membrane cofactor protein; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MGC26544; MIC10; TLX; TRA2.10; trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 50-70 Source/Isotype: Rabbit IgG