{"product_id":"cst-13285t","title":"CST,  13285T, Sara (D5X4F) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SARA. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Sara (D5X4F) Rabbit Monoclonal Antibody (CST #13285) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSara (D5X4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total Sara protein. This antibody also recognizes a non-specific band of unknown origin at 50 kDa.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val488 of human Sara protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Smad anchor for receptor activation (SARA, ZFYVE9) protein is an FYVE domain-containing protein originally identified as a regulator of TGF-β signaling (1). FYVE domains are zinc finger-like domains that bind to phosphatidylinositol 3-phosphate and are responsible for endosomal trafficking (2). While the role of Sara in TGF-β signaling has been questioned (3,4), early research studies demonstrate that Sara enhances TGF-β signaling by binding and recruiting non-activated Smad2 and Smad3 to the TGF-β receptor complex (1). Upon Smad2 activation, Sara dissociates from the complex while phosphorylated Smad2\/3 translocates to the nucleus to bind to the common Smad, Smad4. Sara can also function as an anchor for the protein phosphatase 1 (PP1c) catalytic subunit, which is involved in the Smad7-mediated dephosphorylation of TGF-β type I receptor (5,6). Additional research studies show that expression of Sara plays a critical role in maintenance of the epithelial cell phenotype and that expression is regulated during the epithelial-to-mesenchymal transition (EMT) and fibrosis (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nhSARA; MAD homolog interacting protein; MAD, mothers against decapentaplegic homolog interacting protein, receptor activation anchor; Madh-interacting protein; MADHIP; mothers against decapentaplegic homolog interacting protein; Mothers against decapentaplegic homolog-interacting protein; Novel serine protease; NSP; PPP1R173; protein phosphatase 1, regulatory subunit 173; Receptor activation anchor; SARA; Smad anchor for receptor activation; SMADIP; ZFYV9; ZFYVE9; Zinc finger FYVE domain-containing protein 9; zinc finger FYVE-type containing 9; zinc finger, FYVE domain containing 9\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 190, 210\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797591085225,"sku":"13285T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13285t","provider":"Iright","version":"1.0","type":"link"}