CST, 13287T, Basigin/EMMPRIN (E1S1V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Basigin/EMMPRIN. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Basigin/EMMPRIN (E1S1V) Rabbit Monoclonal Antibody (CST #13287) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 95203 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Basigin/EMMPRIN (E1S1V) Rabbit Monoclonal Antibody recognizes endogenous levels of total EMMPRIN protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala254 of human EMMPRIN protein. Background Basigin (EMMPRIN, CD147) is a type I integral membrane receptor protein belonging to the immunoglobulin superfamily (1). Basigin is a glycosylated protein with four known isoforms, of which isoform 2 is the most abundantly expressed (2). Multiple functions have been ascribed to Basigin; foremost among these is stimulating the secretion of extracellular matrix metalloproteinases by adjacent fibroblasts, a function which has been implicated in promoting tumor progression (2-4). Research studies have shown that Basigin is overexpressed by many tumor cells, and its expression level may correlate with tumor malignancy (5,6). A recent study identified the gene as a regulatory target of Slug, suggesting a role for Basigin in the process of epithelial-mesenchymal transition (7). Basigin has also been identified as a marker for a subset of highly suppressive regulatory T cells (8), and as an obligate receptor for the malarial parasite on human erythrocytes (9). Alternate Names 5F7; BASI; Basigin; basigin (Ok blood group); BSG; CD147; CD147 antigen; Collagenase stimulatory factor; EMMPRIN; EMPRIN; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M6; OK; OK blood group antigen; SLC7A11; TCSF; Tumor cell-derived collagenase stimulatory factor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 38-58 Source/Isotype: Rabbit IgG