{"product_id":"cst-13329s","title":"CST,  13329S, APC1 (D1E9D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying APC1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, APC1 (D1E9D) Rabbit Monoclonal Antibody (CST #13329) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAPC1 (D1E9D) Rabbit Monoclonal Antibody recognizes endogenous levels of total APC1 protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human APC1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nEukaryotic cell proliferation depends strictly upon the E3 ubiquitin ligase activity of the anaphase promoting complex\/cyclosome (APC\/C), whose main function is to trigger the transition of the cell cycle from metaphase to anaphase. The APC\/C complex promotes the assembly of polyubiquitin chains on substrate proteins in order to target these proteins for degradation by the 26S proteasome (1,2). The vertebrate APC\/C complex consists of as many as 15 subunits, including multiple scaffold proteins, two catalytic subunits (APC2, APC11), and a number of proteins responsible for substrate recognition (3). All E3 enzymes, including APC\/C, utilize ubiquitin residues activated by E1 enzymes and transferred to E2 enzymes. Research studies indicate that APC\/C interacts with the E2 enzymes UBE2S and UBE2C via the RING-finger domain-containing subunit APC11 (4-6). APC\/C function relies on multiple cofactors, including an APC\/C coactivator formed by the cell division control protein 20 homolog (CDC20) and Cdh1\/FZR1. The CDC20\/Cdh1 coactivator is responsible for recognition of APC\/C substrates through interaction with specific D-box and KEN-box recognition elements within these substrates (7-9). The ubiquitously expressed anaphase-promoting complex subunit 1 (APC1) is the largest subunit of the APC\/C complex (10). Research studies demonstrate that APC1 undergoes extensive phosphorylation on serine and threonine residues during the mitotic phase of the eukaryotic cell cycle (11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nANAPC1; anaphase promoting complex subunit 1; anaphase-promoting complex 1 (meiotic checkpoint regulator); Anaphase-promoting complex subunit 1; APC1; Cyclosome subunit 1; MCPR; Mitotic checkpoint regulator; Testis-specific gene 24 protein; TSG24\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 216\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797591675049,"sku":"13329S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13329s","provider":"Iright","version":"1.0","type":"link"}