CST, 13359S, CLCN3 (D8Y5Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CLCN3. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, CLCN3 (D8Y5Q) Rabbit Monoclonal Antibody (CST #13359) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Frozen): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen) Specificity / Sensitivity CLCN3 (D8Y5Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total CLCN3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CLCN3 protein. Background Chloride channel 3 (CLCN3) is a voltage-gated chloride channel (CIC) family protein that mediates H /Cl exchange across cell membranes. This 818 amino acid, multi-pass membrane protein is highly expressed in the brain and is especially abundant in the olfactory bulb, hippocampus, and cerebellum (1). CLCN3 protein localizes to endosomal compartments and synaptic vesicles where it contributes to vesicle acidification and proper synaptic vesicle neurotransmitter loading for GABAergic synaptic transmission (2,3). CAMKII-mediated phosphorylation of CLCN3 regulates chloride channel activity by regulating cell surface targeting of the CLCN3 chloride channel (4). Research studies show abnormally high CLCN3 expression at the cell surface of human glioma cells, and that CAMKII-dependent regulation of these channels contributes to glioma invasion (5). Alternate Names chloride channel 3; Chloride channel protein 3; chloride channel, voltage-sensitive 3; Chloride transporter ClC-3; chloride voltage-gated channel 3; ClC-3; CLC3; CLCN3; H(+)/Cl(-) exchange transporter 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 130-150 Source/Isotype: Rabbit IgG