CST, 13375S, TFIIE-alpha Antibody

Polyclonal Antibody for studying TFIIE-alpha. Validated for Western Blotting. Highly specific and rigorously validated in-house, TFIIE-alpha Antibody (CST #13375) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TFIIE-alpha Antibody recognizes endogenous levels of total TFIIE-α protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly243 of human TFIIE-α protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Transcription initiation factor IIE subunit alpha (TFIIE-α) is part of TFIIE, a general transcription factor made up of paired α and β subunits. These general transcription factors include TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH (1-4). Binding of RNA polymerase II (RNAPII) to promoter sequences as part of the pre-initiation complex (PIC) is facilitated by these general transcription factors. These factors also help in selection of the proper transcription start site, DNA unwinding, and RNAPII promoter escape during transcription (1). During the transition from transcription initiation to elongation, TFIIE stimulates the TFIIH kinase and DNA helicase activities, responsible for phosphorylation of the carboxy-terminal domain of the largest RNAPII subunit (POL2RA) and unwinding of promoter DNA for RNAPII promoter escape (1,5-9). Alternate Names FE; General transcription factor IIE 56 kDa subunit; General transcription factor IIE subunit 1; general transcription factor IIE, polypeptide 1, alpha 56kDa; GTF2E1; T2EA; TF2E1; TFIIE alpha subunit; TFIIE-A; TFIIE-alpha; Transcription initiation factor IIE subunit alpha Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit