{"product_id":"cst-13386s","title":"CST,  13386S, UBE1L2\/UBA6 Antibody","description":"Polyclonal Antibody for studying UBE1L2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, UBE1L2\/UBA6 Antibody (CST #13386) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nUBE1L2\/UBA6 Antibody recognizes endogenous levels of total UBE1L2\/UBA6 protein. This antibody does not cross-react with UBE1\/UBA1 or UBE1L\/UBA7 proteins.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys810 of human UBE1L2 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nUbiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thioester complex with the ubiquitin-activating enzyme (E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3). Ubiquitin-activating enzyme E1-like protein 2\/Ubiquitin-like modifier-activating enzyme 6 (UBE1L2\/UBA6) is ubiquitously expressed in human tissues and functions as an E1 enzyme related to UBE1\/UBA1 (40% identity at the protein level). UBE1L2\/UBA6 activates both ubiquitin and the ubiquitin-like protein FAT10 through a similar ATP dependent mechanism (4-6). Like other E1 protein family members, UBE1L2\/UBA6 contains a conserved ATP-binding adenylation domain and an active site cysteine residue that are critical for enzymatic function (4,5). Research studies have demonstrated that UBE1L2\/UBA6 expression is essential during the early stages of embryogenesis in mice (4). Furthermore, loss of neuronal UBE1L2\/UBA6 expression promotes significant defects in neuronal structure and function, which contributes to a reduction in body weight and decreased postnatal viability (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nE1-L2; FLJ10808; FLJ23367; Monocyte protein 4; MOP-4; MOP4; UBA6; UBA6, ubiquitin-activating enzyme E1; UBE1L2; ubiquitin like modifier activating enzyme 6; Ubiquitin-activating enzyme 6; ubiquitin-activating enzyme E1-like 2; Ubiquitin-activating enzyme E1-like protein 2; ubiquitin-like modifier activating enzyme 6; Ubiquitin-like modifier-activating enzyme 6\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 117\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797592789161,"sku":"13386S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13386s","provider":"Iright","version":"1.0","type":"link"}