CST, 13414S, Munc18-1 (D4O6V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Munc18-1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Munc18-1 (D4O6V) Rabbit Monoclonal Antibody (CST #13414) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 76279 . Protocol Available protocols: Western Blotting Specificity / Sensitivity Munc18-1 (D4O6V) Rabbit Monoclonal Antibody recognizes endogenous levels of total Munc18-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr157 of human Munc18-1 protein. Background Munc18-1 belongs to the Munc18 protein family that also includes Munc18-2 and Munc18-3. These proteins are highly conserved at the amino acid sequence level and play a critical role in membrane fusion. Munc18 proteins control SNARE complex formation (1,2) and interact with syntaxin (3). In Munc18-1 knockout mice, neurotransmitter release is completely abolished (4). In addition, Munc18-1 acts as a molecular chaperone for syntaxin-1, allowing for formation of the SNARE complex at the plasma membrane (5). Munc18-1 also acts as a regulator of vesicle docking during exocytosis (6). Alternate Names FLJ37475; MUNC18-1; N-Sec1; neuronal SEC1; NSEC1; p67; Protein unc-18 homolog 1; Protein unc-18 homolog A; RBSEC1; STXB1; STXBP1; syntaxin binding protein 1; Syntaxin-binding protein 1; Unc-18A; UNC18; Unc18-1; UNC18A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG