CST, 13415S, CNOT6 (E1L8F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CNOT6. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CNOT6 (E1L8F) Rabbit Monoclonal Antibody (CST #13415) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CNOT6 (E1L8F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CNOT6 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly400 of human CNOT6 protein. Background The evolutionarily conserved CCR4-NOT (CNOT) complex regulates mRNA metabolism in eukaryotic cells (1). This regulation occurs at different levels of mRNA synthesis and degradation, including transcription initiation, elongation, deadenylation, and degradation (1). Multiple components, including CNOT1, CNOT2, CNOT3, CNOT4, CNOT6, CNOT6L, CNOT7, CNOT8, CNOT9, and CNOT10 have been identified in this complex (2). In addition, subunit composition of this complex has been shown to vary among different tissues (3). The various CNOT proteins display different functions, with CNOT6, CNOT6L, CNOT7 and CNOT8 exhibiting 3'-5' RNAse activity (2,4). Research studies indicate that the CCR4-NOT deadenylase subunits CNOT6 and CNOT6L help mediate cell survival and play a role in cell proliferation, P-body formation, and regulation of gene expression (5). Additional research evidence suggests that CNOT6 can act as a nuclear hormone receptor coactivator that associates with ZNF335 (NIF-1) to regulate expression from specific RARα regulated genes (6). Alternate Names carbon catabolite repression 4 protein; Carbon catabolite repressor protein 4 homolog; CCR4; CCR4 carbon catabolite repression 4-like; CCR4-NOT transcription complex subunit 6; CCR4-NOT transcription complex, subunit 6; CCR4a; CNOT6; Cytoplasmic deadenylase; KIAA1194 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG