{"product_id":"cst-13416s","title":"CST,  13416S, PTMScan® Acetyl-Lysine Motif [Ac-K] Kit","description":"PTMScan for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nCells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan ® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS\/MS analysis. CST recommends the use of PTMScan ® IAP Buffer #9993 included in the kit. 13416 has a higher sensitivity and specificity magnetic bead version: PTMScan ® HS Acetyl-Lysine (Ac-K) Kit in 10-assay (# 46784 ) or 3-assay (#PTMScan ® HS Pilot Acetyl-Lysine Motif (Ac-K) Kit) formats.\n\u003cb\u003eStorage\u003c\/b\u003e\nAntibody beads supplied in IAP buffer containing 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: PTMScan\n\u003cb\u003eBackground\u003c\/b\u003e\nAcetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation\/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of post-translational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nacetyl; Lysine","brand":"CST","offers":[{"title":"Default Title","offer_id":46800792649897,"sku":"13416S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13416s","provider":"Iright","version":"1.0","type":"link"}