CST, 13418S, Rev-Erb-alpha (E1Y6D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Rev-erbα. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Rev-Erb-alpha (E1Y6D) Rabbit Monoclonal Antibody (CST #13418) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Chromatin IP: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Chromatin IP Specificity / Sensitivity Rev-Erb-alpha (E1Y6D) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rev-erbα protein. This antibody may cross-react with an unidentified protein of 60 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser67 of human Rev-erbα protein. Background Reverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins (1). Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells (2). In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm (3-7). Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK-3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock (8). Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2 (9). Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)/HDAC3 complex to target genes to repress transcription (10). Alternate Names EAR-1; EAR1; HREV; NR1D1; nuclear receptor Rev-ErbA-alpha; Nuclear receptor subfamily 1 group D member 1; nuclear receptor subfamily 1, group D, member 1; Rev-erbA-alpha; Rev-ErbAalpha; REVERBA; REVERBalpha; THRA1; THRAL; thyroid hormone receptor, alpha-like; V-erbA-related protein 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG