{"product_id":"cst-13418t","title":"CST,  13418T, Rev-Erb-alpha (E1Y6D) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Rev-erbα. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Rev-Erb-alpha (E1Y6D) Rabbit Monoclonal Antibody (CST #13418) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nChromatin IP: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRev-Erb-alpha (E1Y6D) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rev-erbα protein. This antibody may cross-react with an unidentified protein of 60 kDa.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser67 of human Rev-erbα protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nReverse orientation c-erbA gene α (Rev-erbα, EAR-1, or NR1D1) is a widely expressed member of the orphan nuclear receptor family of proteins (1). Rev-erbα is highly expressed in adipose tissue, skeletal muscle, brain and liver, and regulates cellular proliferation and differentiation. Expression increases during differentiation in adipocytes and ectopic expression of Rev-erbα potentiates the adipocyte differentiation of 3T3-L1 cells (2). In addition, expression oscillates with circadian rhythm in liver cells and Rev-erbα regulates expression of BMAL1, ApoA-I and ApoC-III, all key regulators of circadian rhythm (3-7). Phosphorylation of Rev-erbα Ser55 and Ser59 by GSK-3β appears to stabilize Rev-erbα protein levels and is important for synchronizing and maintaining the circadian clock (8). Rev-erbα also regulates inflammation by targeting the NF-κB responsive genes IL-6 and COX-2 (9). Rev-erbα lacks the activation function 2 domain required for ligand-dependent activation of transcription by other members of the nuclear receptor family; thus it behaves as a constitutive repressor protein, recruiting the nuclear receptor co-repressor (N-CoR)\/HDAC3 complex to target genes to repress transcription (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nEAR-1; EAR1; HREV; NR1D1; nuclear receptor Rev-ErbA-alpha; Nuclear receptor subfamily 1 group D member 1; nuclear receptor subfamily 1, group D, member 1; Rev-erbA-alpha; Rev-ErbAalpha; REVERBA; REVERBalpha; THRA1; THRAL; thyroid hormone receptor, alpha-like; V-erbA-related protein 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 78\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797593510057,"sku":"13418T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13418t","provider":"Iright","version":"1.0","type":"link"}