CST, 13421T, SSRP1 (E1Y8D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SSRP1. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, SSRP1 (E1Y8D) Rabbit Monoclonal Antibody (CST #13421) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity SSRP1 (E1Y8D) Rabbit Monoclonal Antibody recognizes endogenous levels of total SSRP1 protein. This antibody also cross-reacts with protein of unknown origin at 140 kDa in some cell lines. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val145 of human SSRP1 protein. Background Suppressor of Ty-16 (SPT16) and structure-specific recognition protein-1 (SSRP1) are subunits of the facilitates chromatin transcription (FACT) complex that is essential for transcription elongation (1,2). FACT facilitates RNA polymerase-dependent transcription of chromatin templates by destabilizing the nucleosomes within the open reading frames of active genes (3-5). FACT destabilizes the nucleosomes, which would otherwise act as barriers to RNA polymerase transcription activity, by disrupting histone-histone and histone-DNA contacts that lead to the eviction of the histone H2A-H2B dimer (2,3,6). FACT may also function as a histone chaperone to reassemble nucleosomes after RNA polymerase passage (7). In addition to transcription, FACT activity has been shown to have a role in DNA replication in yeast and in DNA repair by contributing to the activation of p53 by CK2 and by facilitating histone H2AX-H2B exchange upon DNA damage (8,9). Alternate Names Chromatin-specific transcription elongation factor 80 kDa subunit; cisplatin-DNA SSRP; facilitates chromatin remodeling 80 kDa subunit; Facilitates chromatin transcription complex 80 kDa subunit; Facilitates chromatin transcription complex subunit SSRP1; FACT; FACT 80 kDa subunit; FACT complex subunit SSRP1; FACT80; FACTp80; high mobility group box; hSSRP1; Recombination signal sequence recognition protein 1; SSRP1; structure specific recognition protein 1; Structure-specific recognition protein 1; T160 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 81 Source/Isotype: Rabbit IgG