{"product_id":"cst-13422t","title":"CST,  13422T, Choline Kinase alpha (D5X9W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CHOK. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Choline Kinase alpha (D5X9W) Rabbit Monoclonal Antibody (CST #13422) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCholine Kinase alpha (D5X9W) Rabbit Monoclonal Antibody recognizes endogenous levels of total choline kinase α protein. Based on the antigen sequence, this antibody is not expected to recognize choline kinase β.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro85 of human choline kinase α protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCholine kinase (ChoK) catalyzes the phosphorylation of choline, a key step in the biosynthesis of the membrane phospholipid phosphatidylcholine. At least three ChoK isoforms exist in mammalian cells, α-1, α-2, and β. The two α isoforms are transcribed from the same gene as splice variants, while the β isoform resides on a separate gene (reviewed in 1). Research studies indicate that ChoKα levels affect signaling through MAPK and Akt pathways (2,3). Investigators have shown that ChoKα plays a role in proliferation and carcinogenesis and is highly expressed\/activated in human cancers (4-7). Additional research studies suggest ChoKα may be a potential target for cancer therapy (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCHETK-alpha; CHK; CHKA; Choline kinase alpha; CK; CKI; EK; Ethanolamine kinase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797593641129,"sku":"13422T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13422t","provider":"Iright","version":"1.0","type":"link"}