CST, 13445T, Securin (D2B6O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying securin. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Securin (D2B6O) Rabbit Monoclonal Antibody (CST #13445) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Securin (D2B6O) Rabbit Monoclonal Antibody recognizes endogenous levels of total securin protein. Based on western blot of overexpressed proteins, this antibody will also recognize securin 2 (PTTG2) to a lesser extent. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val19 of human securin (PTTG1) protein. Background Securin is a regulatory protein that contributes to mitotic checkpoint control, p53 activity, DNA repair, and cell migration. The securin protein prevents premature separation of sister chromatids by inhibiting the protease separase, which cleaves the cohesin complex at the onset of anaphase to allow for sister chromatid segregation (1,2). In mouse oocytes, securin regulates cyclin B stability and G2/M transition (3). Securin negatively affects the transcriptional activity of p53, preventing p53-regulated apoptosis (4). Research studies indicate that securin plays a role in microtubule nucleation, cell polarization, and cell migration (5). Additional research indicates that securin expression may be useful as a biomarker for human breast cancer (6-8). The pituitary tumor-transforming gene 1 ( ) encodes for securin protein, while the highly homologous securin 2 protein is transcribed from the related gene. Securin 2 (PTTG2) protein lacks the ability to inhibit separase activity and may function primarily in cell adhesion and migration (9,10). Alternate Names EAP1; Esp1-associated protein; ESP1-associated protein 1; hPTTG; MGC126883; MGC138276; pituitary tumor-transforming 1; Pituitary tumor-transforming gene 1 protein; PTTG; PTTG1; PTTG1 regulator of sister chromatid separation, securin; Securin; Tumor-transforming protein 1; TUTR1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG