CST, 13452S, HYOU1 Antibody

Polyclonal Antibody for studying HYOU1. Validated for Western Blotting. Highly specific and rigorously validated in-house, HYOU1 Antibody (CST #13452) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HYOU1 Antibody recognizes endogenous levels of total HYOU1 protein. This antibody reacts weakly with murine and rat HYOU1. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human HYOU1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Hypoxia up-regulated protein 1 (HYOU1, ORP150) is an endoplasmic reticulum (ER)-resident stress protein whose expression is upregulated in response to a variety of environmental conditions that promote ER stress (1,2). The amino-terminal region of HYOU1 displays extensive similarity to the ATPase domain of HSP70 family proteins, suggesting that HYOU1 contributes to maintaining proper protein folding and secretion under conditions that promote ER stress (2). Research studies have demonstrated that HYOU1 is cytoprotective and is involved in the inhibition of hypoxia-induced cell death (3). Alternate Names 150 kDa oxygen-regulated protein; 170 kDa glucose-regulated protein; DKFZp686N08236; epididymis secretory sperm binding protein; FLJ94899; FLJ97572; glucose-regulated protein 170; GRP-170; GRP170; HSP12A; HYOU1; hypoxia up-regulated 1; Hypoxia up-regulated protein 1; IMD59; ORP-150; ORP150; oxygen regulated protein (150kD) Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 150 SOURCE: Rabbit