{"product_id":"cst-13483s","title":"CST,  13483S, Sec31A (E1J5M) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Sec31A. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Sec31A (E1J5M) Rabbit Monoclonal Antibody (CST #13483) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSec31A (E1J5M) Rabbit Monoclonal Antibody recognizes endogenous levels of total Sec31A protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1080 of human Sec31A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe coat protein complex II (COPII) is composed of five cytosolic proteins and includes the Sec23\/24 complex, the Sec13\/31 complex, and Sar1. The COPII coat is located at the ER\/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23\/24 complex to form a pre-budding complex that directly binds target molecules (1-3). This pre-budding complex further recruits Sec13\/31 to form mature COPII coat (4,5). The Sec31 subunit of COPII coat interacts with Sec13 at the ER exit and is required for both vesicle formation and ER-Golgi transport. Two isoforms of human Sec31 have been identified, Sec31A and Sec31B, which share a sequence homology of 47.3% (6-8). Sec31A is ubiquitously expressed in tissues and organs, whereas Sec31B is enriched in brain and testis (7,8). In classical Hodgkin lymphoma, a novel fusion of Jak2 with Sec31A renders Jak2 constitutively active and subject to Jak2 inhibitor effects (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nABP125; ABP130; DKFZp686N07171; HSPC275; HSPC334; KIAA0905; MGC90305; NEDSOSB; Protein transport protein Sec31A; protein-transport protein SEC31; SC31A; SEC31 homolog A (S. cerevisiae); SEC31 homolog A, COPII coat complex component; SEC31 homolog A, COPII coating complex component; SEC31-like 1; SEC31-like protein 1; SEC31-related protein A; SEC31A; SEC31L1; Web1-like protein; yeast Sec31p homolog\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 95-140\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797595508905,"sku":"13483S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13483s","provider":"Iright","version":"1.0","type":"link"}