CST, 13489S, NPL4 Antibody

Polyclonal Antibody for studying NPL4. Validated for Western Blotting. Highly specific and rigorously validated in-house, NPL4 Antibody (CST #13489) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NPL4 Antibody recognizes endogenous levels of total NPL4 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys322 of human NPL4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Nuclear protein localization protein 4 (NPL4, NPLOC4) was originally identified as a yeast nuclear transport protein that was later recognized as a critical component of the endoplasmic reticulum-associated degradation (ERAD) pathway (1,2). Mammalian NPL4 protein has an amino-terminal ubiquitin-like domain containing a p97 binding site, and a conserved carboxy-terminal zinc finger (NZF) motif responsible for binding ubiquitinated target proteins (2,3). NPL4 binds ubiquitin fusion degradation protein 1 (UFD1) to form a heterodimer that associates with the p97 AAA-ATPase, creating a protein complex that mediates delivery of ubiquitinated ER proteins to the proteasome (4,5). Alternate Names FLJ20657; FLJ23742; KIAA1499; NPL4; NPL4 homolog, ubiquitin recognition factor; NPLOC4; NPLOC4 ubiquitin recognition factor; nuclear protein localization 4 homolog; nuclear protein localization 4 homolog (S. cerevisiae); Nuclear protein localization protein 4 homolog; Protein NPL4 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 68, 71 SOURCE: Rabbit