{"product_id":"cst-13489s","title":"CST,  13489S, NPL4 Antibody","description":"Polyclonal Antibody for studying NPL4. Validated for Western Blotting. Highly specific and rigorously validated in-house, NPL4 Antibody (CST #13489) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNPL4 Antibody recognizes endogenous levels of total NPL4 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys322 of human NPL4 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nNuclear protein localization protein 4 (NPL4, NPLOC4) was originally identified as a yeast nuclear transport protein that was later recognized as a critical component of the endoplasmic reticulum-associated degradation (ERAD) pathway (1,2). Mammalian NPL4 protein has an amino-terminal ubiquitin-like domain containing a p97 binding site, and a conserved carboxy-terminal zinc finger (NZF) motif responsible for binding ubiquitinated target proteins (2,3). NPL4 binds ubiquitin fusion degradation protein 1 (UFD1) to form a heterodimer that associates with the p97 AAA-ATPase, creating a protein complex that mediates delivery of ubiquitinated ER proteins to the proteasome (4,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ20657; FLJ23742; KIAA1499; NPL4; NPL4 homolog, ubiquitin recognition factor; NPLOC4; NPLOC4 ubiquitin recognition factor; nuclear protein localization 4 homolog; nuclear protein localization 4 homolog (S. cerevisiae); Nuclear protein localization protein 4 homolog; Protein NPL4\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 68, 71\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797595574441,"sku":"13489S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13489s","provider":"Iright","version":"1.0","type":"link"}