CST, 13492T, Atg101 (E1Z4W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ATG101. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Atg101 (E1Z4W) Rabbit Monoclonal Antibody (CST #13492) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Atg101 (E1Z4W) Rabbit Monoclonal Antibody recognizes endogenous levels of total Atg101 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val177 of human Atg101 protein. Background Atg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase complex required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes, including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2). Alternate Names ATG101; Atg13-interacting protein; ATGA1; autophagy related 101; Autophagy-related protein 101; C12orf44; chromosome 12 open reading frame 44; FLJ11773 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG