{"product_id":"cst-13492t","title":"CST,  13492T, Atg101 (E1Z4W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ATG101. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Atg101 (E1Z4W) Rabbit Monoclonal Antibody (CST #13492) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAtg101 (E1Z4W) Rabbit Monoclonal Antibody recognizes endogenous levels of total Atg101 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val177 of human Atg101 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAtg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase complex required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes, including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nATG101; Atg13-interacting protein; ATGA1; autophagy related 101; Autophagy-related protein 101; C12orf44; chromosome 12 open reading frame 44; FLJ11773\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 25\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797595705513,"sku":"13492T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13492t","provider":"Iright","version":"1.0","type":"link"}