CST, 13517T, IL-2R alpha/CD25 (D6K5F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL2RA. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-2R alpha/CD25 (D6K5F) Rabbit Monoclonal Antibody (CST #13517) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IL-2R alpha/CD25 (D6K5F) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-2Rα (CD25) protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala34 of human IL-2Rα (CD25) protein. Background Interleukin-2 (IL-2) is a T cell stimulatory cytokine best known for inducing T cell proliferation and NK cell proliferation and activation (1,2). IL-2 also promotes peripheral development of regulatory T cells (Tregs) (3,4). Conversely, IL-2 is involved in the activation-induced cell death (AICD) that is observed post T cell expansion by increasing levels of Fas on CD4 T cells (5). The effects of IL-2 are mediated through a trimeric receptor complex consisting of IL-2Rα, IL-2Rβ, and the common gamma chain, γc (1,2). IL-2Rα binds exclusively to IL-2 with low affinity and increases the binding affinity of the whole receptor complex including IL-2Rβ and γc subunits. IL-15 also binds to IL-2Rβ (1,2). γc is used by other cytokines, including IL-4, IL-7, IL-9, IL-15, and IL-21 (1,2). Binding of IL-2 initiates signaling cascades involving Jak1, Jak3, Stat5, and the PI3K/Akt pathways (1,2). Alternate Names CD25; IDDM10; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; IL2R; IL2RA; IMD41; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; Interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35, 60 Source/Isotype: Rabbit IgG