CST, 13525S, Rad23B (D4W7F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RAD23B. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Rad23B (D4W7F) Rabbit Monoclonal Antibody (CST #13525) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Rad23B (D4W7F) Rabbit Monoclonal Antibody recognizes endogenous levels of total Rad23B protein. This antibody does not cross-react with Rad23A protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala140 of human Rad23B protein. Background The yeast nucleotide excision repair (NER) radiation sensitive protein 23 (rad23) and its human homologs Rad23A (hHR23A) and Rad23B (hHR23B) are critical components of the cellular machinery that recognize DNA lesions and serve as receptors that target ubiquitinated substrates to the proteasome for degradation (1). The UV excision repair protein Rad23B is a multi-domain scaffold protein that plays an important role in ubiquitin-dependent proteasomal degradation. Rad23B contains an amino-terminal ubiquitin-like (UbL) domain that facilitates interaction with the S5a/PSMD4 subunit of the proteasome 19S regulatory complex (2,3). In addition, Rad23B contains a central ubiquitin-associated domain (UBA1) and a carboxy-terminal UBA2 domain, which bind mono- and polyubiquitin with distinct specificities (4). Research studies demonstrate that Rad23B binds specifically to K48-ubiquitinated proteins to facilitate recruitment of target proteins to the proteasome (5). Between the paired UBA domains, Rad23B contains an XPC-binding domain that facilitates binding to XPC and recruitment to DNA lesions (6), as well as the binding of peptide:N-glycanase that is critical for recruitment of ubiquitinated ERAD substrates to the proteasome (7). Research studies have shown that targeted deletion of the murine locus impairs embryonic development, suggesting that Rad23B is essential for mammalian development (8). Alternate Names hHR23B; HR23B; p58; RAD23 homolog B (S. cerevisiae); RAD23 homolog B, nucleotide excision repair protein; RAD23, yeast homolog of, B; RAD23B; RD23B; UV excision repair protein RAD23 homolog B; XP-C repair complementing complex 58 kDa; XP-C repair complementing protein; XP-C repair-complementing complex 58 kDa protein Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 53 Source/Isotype: Rabbit IgG