CST, 13531S, Afadin (D1Y3Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Afadin. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Afadin (D1Y3Z) Rabbit Monoclonal Antibody (CST #13531) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Afadin (D1Y3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total afadin protein. Based on the protein sequence, this antibody is expected to recognize all afadin isoforms. Species Reactivity: Human, Mouse, Rat, Monkey, Dog Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg1117 of human afadin protein. Background In multicellular organisms, intercellular junctions play essential roles in tissue integrity and maintenance of cell polarity. Tight junctions (TJs) form a continuous barrier to fluids across the epithelium and endothelium (reviewed in 1). Adherens junctions (AJs) are dynamic structures that form cell-cell contacts linking cells into a continuous sheet (reviewed in 2). The actin filament-binding protein, Afadin, binds to nectin forming a connection to the actin cytoskeleton (3). AJs are formed when nectin assembles cadherin at the cell-cell adhesion site and these junctions are then involved in the formation and maintenance of TJs (4,5). Afadin has two splice variants: l-afadin, which is ubiquitously expressed, and s-afadin, which is expressed predominantly in neural tissue. s-Afadin is a shorter form lacking one of the three proline-rich regions found in l-afadin, as well as the carboxyl-terminal F-actin binding region (6). Human s-afadin is identical to AF-6, the ALL-1 fusion partner involved in acute myeloid leukemias (7). Recent work has also shown that afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells (8,9). Alternate Names AF-6; AF6; AFAD; Afadin; Afadin adherens junction formation factor; afadin, adherens junction formation factor; AFDN; ALL1-fused gene from chromosome 6 protein; FLJ34371; l-afadin; MLL-AF6; MLLT4; myeloid/lymphoid or mixed-lineage leukemia; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); myeloid/lymphoid or mixed-lineage leukemia, translocated to, 4; Protein AF-6; RP3-431P23.3; translocated to, 4 Specification REACTIVITY: H M R Mk Dg SENSITIVITY: Endogenous MW (kDa): 205 Source/Isotype: Rabbit IgG