{"product_id":"cst-13566t","title":"CST,  13566T, Phospho-Na,K-ATPase alpha1 (Tyr10) (E1Y9C) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ATP1A1 (Tyr10) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Na,K-ATPase alpha1 (Tyr10) (E1Y9C) Rabbit Monoclonal Antibody (CST #13566) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Na,K-ATPase alpha1 (Tyr10) (E1Y9C) Rabbit Monoclonal Antibody recognizes endogenous levels of Na,K-ATPase α1 protein only when phosphorylated at Tyr10. This antibody cross-reacts with an induced 75-80 kDa doublet of unknown origin.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr10 of human Na,K-ATPase α1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit (reviewed in 1). Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1\/2 phosphorylation of the α subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAT1A1; ATP1A1; ATPase Na+\/K+ transporting subunit alpha 1; ATPase, Na+\/K+ transporting, alpha 1 polypeptide; CMT2DD; HOMGSMR2; MGC3285; MGC51750; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; Na(+)\/K(+) ATPase alpha-1 subunit; Na+, K+ ATPase alpha subunit; Na+\/K+ ATPase 1; sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; Sodium\/potassium-transporting ATPase subunit alpha-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797597081769,"sku":"13566T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-13566t","provider":"Iright","version":"1.0","type":"link"}